A cross-neutralizing antibody between HIV-1 and influenza virus
نویسندگان
چکیده
Incessant antigenic evolution enables the persistence and spread of influenza virus in human population. As principal target immune response, hemagglutinin (HA) surface antigen on viruses continuously acquires replaces N -linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans. Anti-glycan antibodies, such as 2G12, HIV-1 envelope (Env), which is even more extensively glycosylated contains under-processed oligomannose-type clusters its dense glycan shield. Here, we illustrate that 2G12 can also neutralize seasonal A H3N2 have evolved present similar their HAs from around 20 years after 1968 pandemic. Using structural biology mass spectrometric approaches, find two -glycosylation close receptor binding site (RBS) represent oligomannose cluster recognized by 2G12. One these highly conserved all strains other emerged during evolution. These become crucial for fitness recent strains. findings shed light suggest 2G12-like antibodies potentially act broad neutralizers enveloped viruses.
منابع مشابه
HIV-1 Cross-Reactive Primary Virus Neutralizing Antibody Response Elicited by Immunization in Nonhuman Primates.
Elicitation of broadly neutralizing antibody (bNAb) responses is a major goal for the development of an HIV-1 vaccine. Current HIV-1 envelope glycoprotein (Env) vaccine candidates elicit predominantly tier 1 and/or autologous tier 2 virus neutralizing antibody (NAb) responses, as well as weak and/or sporadic cross-reactive tier 2 virus NAb responses with unknown specificity. To delineate the sp...
متن کاملExploiting Natural Cross-reactivity between Human Immunodeficiency Virus (HIV)-1 p17 Protein and Anti-gp41 2F5 Antibody to Induce HIV-1 Neutralizing Responses In Vivo
Anti-p17 antibodies are able to neutralize human immunodeficiency virus (HIV) entry in a mouse model. In this study, we identified a region of sequence similarity between the epitopes of anti-p17 neutralizing antibodies and anti-gp41 neutralizing 2F5 antibody and verified cross-reactivity between p17 and 2F5 in vitro. The p17 sequence was modified to increase sequence identity between the p17 a...
متن کاملFactors affecting the bluetongue virus neutralizing antibody response and the reaction between virus and antibody.
A study was made of different aspects of the bluetongue virus neutralizing antibody response and the reaction between the virus and antibody. Optimum neutralization was obtained in a 2mM Tris-HCl buffer, pH 9,0, at a temperature of 4 degrees C. The reaction of virus and antibody could be demonstrated by electron microscopy in the formation of clumps which were shown to be serotype specific. It ...
متن کاملHIV-1 Dual Infected LTNP-EC Patients Developed an Unexpected Antibody Cross-Neutralizing Activity
This study evaluated the neutralization breadth in dually infected (DI) HIV-1 long-term non-progressor elite controller patients (LTNP-EC) using a representative minipanel of 6 viruses from 5 different subtypes. Our results showed an improved neutralization breadth in DI LTNP-EC patients when compared with matched LTNP single-infected patients. The role of viral diversity in neutralization was ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLOS Pathogens
سال: 2021
ISSN: ['1553-7366', '1553-7374']
DOI: https://doi.org/10.1371/journal.ppat.1009407